Is it necessary to remove MTT medium prior to DMSO addition?

Is it necessary to remove MTT medium prior to DMSO addition?

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Is it necessary to remove MTT medium prior to DMSO addition?

Removal of MTT should not be done because whatever formazan crystals have been ... We just make sure to spin the cells down before media removal. ... Morever, it is expected to always run standard curves with graded cell densities and this ...

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