Immunohistochemistry - WormBook

Immunohistochemistry - WormBook

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Immunohistochemistry - WormBook

For a discussion of electron microscopy and immuno-EM in C. elegans (not covered in this section), see the ... Anterior to left, ventral down, scale bar is 10 μm.

IHC Tests (ImmunoHistoChemistry) | Breastcancer.org

21 Jan 2020 ... IHC is used to show whether or not the cancer cells have HER2 receptors and/or hormone receptors on their surface. This information plays a ...

Wormbook.org

WormBook is a comprehensive, open-access collection of original, peer-reviewed chapters covering topics related to the biology of Caenorhabditis elegans and ...

Immunohistochemistry Principle & Protocol - Boster Bio

Since xylene has a span and fast contractility to tissue, the tissue should not be ... the tissue in a paraffin box so that the paraffin wax cools down and solidifies.

Immunohistochemistry Guide - Creative Diagnostics

This is not achievable, as the alteration in chemical structure caused by fixation ... slows down subsequently), and is often recommended for nucleic acid work.

Behavior - WormBook

Animals clearly behave differently depending on their feeding status and history. ... Most C. elegans laboratories that specialize in behavior have their own “horror stories” in which genetic drift due to continuous passaging or ... R-project.org).

Colorectal Cancer Antibodies used in Immunohistochemistry | LSBio

BRAF V600E is a pro-proliferative mutation, and while the adoption of V600E-mutant BRAF protein alone is not a pathogenic event (this mutation is the source of ...

The value of immunohistochemistry in sentinel lymph node ... - NCBI

7 Jun 2005 ... Immunohistochemistry was not performed on lymph nodes obtained ... the smallest metastatic deposits down to single isolated tumour cells.

LSBio: Leaders in Immunohistochemistry (IHC) Validated Antibodies ...

LSBio is the trusted leader in highly sensitive and specific IHC Validated Antibodies, ELISA Kits, Proteins and Nucleic Acids.

Maintenance of C. elegans - WormBook

Wild-type and over 3,000 mutant strains of C. elegans are available at no cost to researchers ... This cuts down on contamination. ... The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.101.1, http://www.wormbook.org.

Reverse genetics - WormBook

Finally, the vast majority of genes have not yet been mutated in most ... Using RT-PCR, you can test whether the mRNA has been knocked down. ... at: http://celeganskoconsortium.omrf.org/ or http://shigen.lab.nig.ac.jp/c.elegans/ index.jsp.

10 Tips for Successful Immunohistochemistry - Enzo Life Sciences

Knock-out or knock-down tissue samples are recommended. They should be used alongside a no-primary control as well as an isotype control. Our high sensitivity ...

Web resources for C. elegans studies - WormBook

It does not contain a comprehensive list of Web sites and services since links to other ... WormBase <http://www.wormbase.org> is a major repository for C. elegans ... query box, select an information type from the pull down menu to the left and ...

Keeping track of worm trackers - WormBook

C. elegans is used extensively as a model system in the neurosciences due to its ... (under a cover slip, effectively slowing down but not preventing locomotion). ... a fully integrated GUI (http://farsight-toolkit.org/wiki/Worm_Analysis_System and ...

Genetic mapping and manipulation: Chapter 1 ... - WormBook

On the down side, genetics can sometimes seem like a slow and arduous ... In this sense, C. elegans genetics is not substantially different from many other ...

Getting into the mind of a worm—a personal view - WormBook

My parents thought that this trait was not to be encouraged when applied to living ... to obtain a detailed knowledge of the synaptic connections of C. elegans. ... that Eileen arrived on my scene, Sydney started to wind down his own anatomical studies. ... WormBook, doi/10.1895/wormbook.1.158.1, http://www.wormbook.org.

A Transparent window into biology: A primer on ... - WormBook

18 Jun 2015 ... These possibilities make C. elegans useful not only in research ... Images modified from those found at www.wormatlas.org (Altun et al. ... in hermaphrodites and down-regulates X-linked genes by 50% to equal the gene ...

A biologist's guide to statistical thinking and analysis - WormBook

In some cases, standard numerical summaries (e.g., mean and SD) may not be ... Remember: SD is variation among individuals, SE is the variation for a ... Many studies in our field boil down to generating means and comparing them to each ...

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