FROM CELL SIGNALING TECHNOLOGY | www.cellsignal.com ... is not sufficient to guarantee a chosen antibody will perform in IF (see figure on this page).
www.cellsignal.com/support (USA & Europe) | www.cst-c.com.cn/support (China) | www.cstj.co.jp/support (Japan). Production Team ... Tween® 20 should not be present in the Blocking Buffer because it is auto-fluorescent and increases non-specific ... Scrape cells off the plate and transfer to microcentrifuge tubes. Keep on ...
Italy (€) EUR; ja-JP ... IF can further be broken down into two categories: direct and indirect. ... Depending on the cell type, the cells may not adhere to the coverslip on their own and the coverslips will need to be coated ... Immunohistochemistry is most commonly performed on tissue samples that have either been frozen in a ...
Methanol fixed samples do not require permeabilization. ... Using a pair of forceps lower the cover slip down onto the mounting medium allowing the liquid to ...
